Tools and training
The current limitation for biologists has been the increasing demand for computational power and training required for processing of NGS data. We developed the Brazilian Microbiome Project Operating System (BMPOS), a flexible and user-friendly Linux distribution dedicated to microbiome studies. The BMPOS is freely available and possesses the entire requirement of bioinformatics packages and databases to perform all the pipelines suggested by the BMP team.
BTW—BIOINFORMATICS THROUGH WINDOWS
We developed a fully automated and easy-to-install package as well as a complete, easy-to-follow pipeline for microbial metataxonomic analysis operating in the Windows Subsystem for Linux (WSL)—Bioinformatics Through Windows (BTW). BTW combines several open-access tools for processing marker gene data, including 16S rRNA, bringing the user from raw sequencing reads to diversity-related conclusions. It includes data quality filtering, clustering, taxonomic assignment and further statistical analyses, directly in WSL, avoiding the prior need of migrating from Windows to Linux.
PIME - PREVALENCE INTERVAL FOR MICROBIOME EVALUATION
PIME removes the within group variation found in metataxonomic surveys (16S rRNA datasets) by capturing only biological differences at high sample prevalence levels.